Figure 1: Development of xenogeneic immune cells in NSG mice.
A: Newborn mice are transplanted with human hematopoietic stem cells. Peripheral blood is analyzed by means of flow cytometric analysis at different time points after transplantation. The left dot plot shows leukocytes of a successfully transplanted mouse at 15 weeks after transplantation. Human and mouse leukocytes can be differentiated. Within all living leukocytes, 35.4 % are murine and 53.8 % have human origin. In the right dot plot human CD45+ cells are plotted against sideward scatter (SSC). Population (a) represents lymphocytes whereas population (b) are monocytes and population (c) represents granulocytes.
B: Percentages of huCD45+ subpopulations differ during time course after transplantation. Dot plots depict representative percentages of huCD19+ and huCD3+ within population of huCD45+. Ten weeks old animals show higher levels of huCD19+ compared to 15 weeks old animals but a lower amount of huCD3+ in comparison to the older mice.
C: Flow cytometric analysis of peripheral blood in the time course after transplantation of a newborn NSG mouse with bovine hematopoietic stem cells. Percentages of bovine CD45+ cells substantially increase with time after transplantation.