Bacterial respiratory infection rodent models


Bacterial respiratory infection rodent models, figure 1.


Bacterial respiratory infection rodent models, figure 2.

Bacterial pulmonary infections are a major problem in immunocompromised patients. Due to increasing emergence of antibiotic resistance the need to discover new antimicrobial therapeutics increases drastically.

At Fraunhofer ITEM, mouse models of bacterial pulmonary infection are established e.g. for S. aureus (acute) and P. aeruginosa (chronic). In acute infection models, main read outs are health score/ survival, bacterial load in lung and peripheral tissues, inflammatory cell counts. In chronic infection model, the health score, inflammatory response and particularly persistence of the pathogen are main readouts.

Species

Mouse, Rat

Fields of application

  • preclinical efficacy testing

Endpoints/Outcome parameter

  • Bacterial pulmonary infection
  • Host response

Readout parameter

  • Lung function measurements possible
  • Bronchoalveolar lavage: cell count + differential cell count (incl. inflammatory cells)
  • Bacterial load in BAL and lung tissue, dissemination to other organs
  • Cytokine profiles (ELISA, MSD)
  • Histology: conventional staining and tissue pathohistology/conventional microscopy (e.g. tissue cell infiltration), immunohistochemistry/fluorescence microscopy, confocal microscopy (e.g. for detection of targets e.g. receptor expression)
  •  Lung tissue analysis (RNA isolation/ gene expression, cytokine profiles,…)
  • Flow cytometric analysis

Quality management and validation

  • Internal quality management
  • Historical data available
  • Reference compounds e.g. dexamethasone, fluticasone
  • Performance under GLP possible