Adherent cell lines
This assay is used to test the viability of cells after incubation with small molecules and to determine the toxic effect of respective substances. Damaged cells release the intracellular enzyme lactate dehydrogenase which can be determined using a fluorometric assay. The amount of enzyme activity correlates with the degree of toxicity of the substances.
Three different cell lines are mainly used:
This model can be used for the following fields of application:
Percentage of the viability of cells after substance incubation compared to untreated control cells.
Fluorometric measurement of lactate dehydrogenase activity.