House dust mite (HDM)- induced severe chronic asthma model


House dust mite (HDM)- induced severe chronic asthma model.

House dust mite (HDM) is a common allergen associated with allergic asthma in humans. The HDM mouse model of chronic allergic asthma is a state of the art asthma model, reflecting closely the local administration route (intranasal) without adjuvants. The consecutive exposure to HDM over several weeks leads to induction of chronic allergic asthma phenotype, with eosinophil/Th2 dominant phenotype but also presence of neutrophils, a pronounced airway hyperresponsiveness and remodeling in the lung tissue.

Species

Mouse

Fields of application

  • preclinical efficacy testing

Endpoints/Outcome parameter

  • Allergic Airway Inflammation & Airway Hyperresponsivenss
  • Exacerbation

Readout parameter

  • Lung function measurements e.g. airway hyperresponsiveness (AHR)
  • Bronchoalveolar lavage: cell count + differential cell count (incl. inflammatory cells), cytokine profiles,
  • Cytokine profiles (ELISA, MSD)
  • Histology: conventional staining and tissue pathohistology/conventional microscopy (e.g. tissue cell infiltration), immunohistochemistry/fluorescence microscopy, confocal microscopy (e.g. for detection of targets e.g. receptor expression)
  • Lung tissue analysis (RNA isolation/ gene expression, cytokine profiles,…)
  • Flow cytometric analysis

Quality management and validation

  • Internal quality management
  • Historical data available
  • Reference compounds e.g. dexamethasone, fluticasone
  • Performance under GLP possible

References

  • This animal model was established in frame of the U-BIOPRED project in cooperation with GSK.
  • Rochlitzer S, Hoymann HG, Müller M, Braun A; U-BIOPRED consortium. Clin Sci (Lond). 2014 Jan 1;126(1):55-65.

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