LPS - induced respiratory inflammation in rodents


LPS - induced respiratory inflammation in rodents.

LPS-induced respiratory inflammation is an established model in the mouse and rat. It is a robust, easy and cheep model that can be used especially for efficacy testing of anti-inflammatory compounds.

We have established local administration of LPS via intratracheal, intranasal or inhalative route. The models available use single LPS application (acute model) or consecutive low dose LPS challenges (prolonged model).

Species

Mouse, Rat

Fields of application

  • preclinical efficacy testing

Endpoints/Outcome parameter

  • Acute Airway Inflammation

Readout parameter

  • Lung function measurements
  • Bronchoalveolar lavage: cell count + differential cell count (incl. inflammatory cells)
  • Cytokine profiles (ELISA, MSD)
  • Histology: conventional staining and tissue pathohistology/conventional microscopy (e.g. tissue cell infiltration), immunohistochemistry/fluorescence microscopy, confocal microscopy (e.g. for detection of targets e.g. receptor expression)
  • Lung tissue analysis (RNA isolation/ gene expression, cytokine profiles,…)
  • Flow cytometric analysis

Quality management and validation

  • Internal quality management
  • Historical data available
  • Reference compounds e.g. dexamethasone, roflumilast
  • Performance under GLP possible