Melanoma


Melanoma skin model generated from human primary keratinocytes and a melanoma cell line. Melanoma cells are indicated in brown.

To mimic a primary skin melanoma, melanoma cells can be introduced into the Open-Source Reconstructed Epidermis. In this model the melanoma cells form tumor equivalents that recapitulates the three-dimensional structure of a primary tumor within the microenvironment of the human epidermis.

Species

Human

Fields of application

  • Efficacy of tumor therapeutics
  • Melanoma cell - keratinocyte interaction

Endpoints/Outcome parameter

  • Viability of keratinocytes and melanoma cells
  • Cellular interactions
  • Genetic profile

Readout parameter

  • MTT measurements
  • Live cell imaging
  • Real-time polymerase chain reaction