Model of acute kidney injury (AKI) induced by cisplatin in mice

acute kidney injury, cisplatin
Figure 1: DAB-staining of a target protein involved in acute kidney failure (brown color). Frozen sections of murine kidney tissue. Magnification: 20x.

Species

Mouse

Field of application:

AKI is induced by a single i.p. application of cisplatin. Mice are housed in metabolic cages up to 72 hours after induction to collect urine and faeces.

Model can be used for the following fields of application:

  • Pharmacodynamics and pharmacokinetics
  • (Patho)physiological processes
  • Therapeutic efficacy
  • Proof of concept

Endpoints/Outcome parameter

  • Serum creatinine and urea
  • Urine analysis
  • Immunohistochemistry
  • Histology

Readout parameter

  • Body weight
  • Food and water intake
  • Serum creatinine and urea
  • Urine analysis
  • Western blot (urine)
  • Histology (PAS and TUNEL staining, leucocyte infiltration)
  • Immunohistochemistry

Quality management and validation

  • Controls
  • Randomisation
  • Blinded data analysis
  • Internal quality management

Weblink(s)

https://www.izi.fraunhofer.de/de/departments/halle-location/department-of-drug-design-and-target-validation/molecular-biotechnology.html#tabpanel-2

References

  • Malhotra R, Siew ED. Biomarkers for the Early Detection and Prognosis of Acute Kidney Injury. Clin J Am Soc Nephrol. 2017 Jan 6; 12(1): 149–173.