Mouse model for infectious disease (GLP-like / GLP)


Fig.: Mice are infected with Salmonella enterica to induce systemic infection. The course of infection is analyzed using various approaches, e.g. flow cytometric analysis of immune cell subtypes in different organs.



Field of application

Salmonella enterica causes various diseases including food poisoning and typhoid fever. We provide a mouse model for infectious disease using Salmonella enterica to test antibacterial agents and potential therapeutics. Furthermore, immunomodulatory / immunotoxic effects of test substances can be revealed by infecting mice with a sublethal dose of Salmonella enterica and simultaneous exposure to the test substance. Mice can also be infected with a median lethal (LD50) infection dose to determine the impact of test substances on mortality. The model can thus be used as therapy and / or toxicity model.

  • Diagnosis and therapy model for infections with Salmonella enterica
  • Testing of antibacterial agents and potential therapeutics
  • Testing of immunomodulatory / immunotoxic substances
  • Evaluation of application/treatment protocols for therapeutic agents
  • Efficacy and safety studies for drug approval (non-GLP and GLP)

Endpoints/Outcome parameter

  • Survival rate
  • Cytokine / antibody status in serum
  • Bacterial burden in peritoneum and different organs
  • Phenotyping of infiltrating inflammatory cells in spleen, lymph nodes and peritoneum
  • Functional activity of different immune cell subtypes (phagocytosis, NO production, cytokine / antibody response etc.)

Readout parameter

  • Daily evaluation of clinical symptoms
  • Periodic blood sampling
  • Flow cytometric analysis / Cell sorting / Imaging flow cytometry
  • ELISA / Multiplex analysis
  • Quantitative real-time PCR
  • Histology
  • Immunohistochemistry / Immunofluorescence

Quality management measures

  • Internal quality management (certified GLP test facility)
  • Use of reference compounds (Standard-of-care therapies)
  • Randomisation
  • Blinded induction, data acquisition and analysis
  • Biometric expertise


Lehmann J, Bellmann S, Werner C, Schröder R, Schütze N, Alber G. IL-12p40-Dependent Agonistic Effects on the Development of Protective Innate and Adaptive Immunity Against Salmonella Enteritidis. J Immunol. 2001 Nov 1;167(9):5304-15

Lehmann J, Springer S, Werner CE, Lindner T, Bellmann S, Straubinger RK, Selbitz HJ, Alber G. Immunity induced with a Salmonella enterica serovar Enteritidis live vaccine is regulated by Th1-cell-dependent cellular and humoral effector mechanisms in susceptible BALB/c mice. Vaccine. 2006 May 29;24(22):4779-93. Epub 2006 Mar 29