Mouse models for inflammatory bowel disease (GLP-like / GLP)

Fig. 1: Tissue architecture of the distal colon in a healthy animal (left) and in chronic DSS-induced colitis (right). Scale bar: 500 µm


Fig. 2: Expression of Immunoglobulin A (red) and T helper cells (CD4; green) in the distal colon of an animal with chronic DSS-induced colitis. Scale bar: 100 µm



Field of application

Inflammatory bowel diseases (IBD), including Crohn’s disease and ulcerative colitis, are chronic-relapsing disorders of the gastrointestinal tract with multifactorial pathogenesis. The model of dextran sulfate sodium (DSS)-induced colitis is a widely used animal model in basic and translational IBD research. We have developed a refined model of chronic DSS-induced colitis that reflects typical disease symptoms and features high reproducibility. The model is therefore ideally suited for evaluating the efficacy of new IBD therapies.

  • Testing of potential therapeutics for IBD
  • Evaluation of application/treatment protocols for therapeutic agents
  • Efficacy studies for drug approval

Endpoints/Outcome parameter

  • Clinical Score
  • Imaging - Monitoring of gut wall lesions
  • Gut permeability
  • Histopathological Score and colon length
  • Profile of immune cells and/or inflammatory markers (e.g. cytokines) in colon tissue
  • Profile of microbiota composition
  • Expression of disease-relevant tissue markers (e.g. tight junction proteins)

Readout parameter

  • Daily evaluation of clinical parameters
  • Small animal endoscopy
  • FITC-Dextran test
  • Histology
  • Immunohistochemistry / Immunofluorescence
  • Flow cytometric analysis / Cell sorting
  • ELISA / Multiplex analysis
  • 16S rRNA sequencing

Quality management measures

  • Internal quality management (certified GLP test facility)
  • Use of reference compounds (Standard-of-care therapies)
  • Randomisation
  • Blinded induction, data acquisition and analysis
  • Biometric expertise

Quality management measures

Hoffmann M, Schwertassek U, Seydel A, Weber K, Falk W, Hauschildt S, Lehmann J (2017). A refined and translationally relevant model of chronic DSS colitis in BALB/c mice. Lab Anim. 2017 Jan 1:23677217742681 [Epub ahead of print]

Hoffmann M, Schwertassek U, Seydel A, Weber K, Hauschildt S, Lehmann J. Therapeutic efficacy of a combined sage and bitter apple phytopharmaceutical in chronic DSS-induced colitis. Sci Rep. 2017 Oct 27;7(1):14214. doi: 10.1038/s41598-017-13985-x