Ovalbumin-induced acute asthma model in mice and rats


The Ova mouse model of acute allergic asthma is a well-established asthma model. Mice or Brown-Norway rats will be sensitized systemically and challenged inhalationally with ovalbumin. During the challenge the early airway response (EAR) and 4-5 h later the late airway response (LAR) can be registered by lung function measurement. After 24 h or 48 h, using a second lung function measurement, the airway hyperresponsiveness will be registered followed by a bronchoalveolar lavage with immunologic and histologic analyses of the inflammatory allergic response. Exact individual dosing during inhalation provocation or drug treatments will be achieved by means of a computerized feedback dose-control system.


Mouse, rat

Fields of application

  • Preclinical efficacy testing

Endpoints/Outcome parameter

  • Early Airway Response (EAR)
  • Allergic Airway Inflammation & Airway Hyperresponsiveness (AHR)Tumor (patho)histology (ex vivo)

Readout parameter

  • Lung function measurements e.g. early airway response (EAR) and airway hyperresponsiveness (AHR)
  • Bronchoalveolar lavage: cell count + differential cell count (incl. inflammatory cells), cytokine profiles,
  • Cytokine profiles (ELISA, MSD)
  • Histology: conventional staining and tissue pathohistology/conventional microscopy (e.g. tissue cell infiltration), immunohistochemistry/fluorescence microscopy, confocal microscopy (e.g. for detection of targets e.g. receptor expression)
  • Lung tissue analysis (RNA isolation/ gene expression, cytokine profiles,…)
  • Flow cytometric analysis

Quality management and validation

  • Internal quality management
  • Historical data available
  • Reference compounds e.g. dexamethasone, fluticasone

Performance under GLP possible


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