Figure 1: Symptoms of sepsis in humanized NSG mice.
A+B: Histological sections of spleens stained with H&E (Hematoxylin and Eosin stain).
A shows a spleen of a representative mouse treated with saline. Regularly developed white pulp with lymphocytic follicles is visible (asterisk) sinusoids are covered by lymphocytes (arrow) and few erythrocytes.
B: Spleen of an animal treated with 5 mg/kg LPS. The mouse was euthanized 6 hours after treatment. White pulp is extremely diminished while red pulp dominates. Lymphocytic follicles are decreased in size and erythrocytes fill the space between sinusoids.
C+D: The levels of both human and murine TNFα and IL-6 in blood plasma were analyzed by cytometric bead array. All cytokines are strongly increased 6 hours after injection of LPS compared to control animals injected with saline. ** p ≤ 0.01; *** p ≤ 0.001.