Field of application:
Thioglycolate causes a local inflammation after i.p. application. After 4 hours a peritoneal lavage using PBS is done. Lavage fluid is analyzed by FACS and the infiltration of immune cells can be quantified. Lavage fluid can also be analyzed by Multiplex analysis or ELISA. The method can be used to investigate the influence if small molecules or biologicals on cell invasion and on different inflammatory parameters.
The model can be used for the following fields of application:
- Identification of immune cells (Monocytes, Granulocytes, etc.)
- Quantification of inflammatory parameters (Cytokines, etc.)
Quality management and validation
- Non GLP
- Internal quality management
- (1) Cynis et al. The isoenzyme of glutaminyl cyclase is an important regulator of monocyte infiltration under inflammatory conditions. EMBO molecular medicine. 2011; 9: 545–558.