Fraunhofer-Verbund Life Sciences
Fraunhofer-Verbund Life Sciences

Thioglycolate-induced Peritonitis in DBA/1 mice

in vivo screening
Figure 1: Scatter plot showing the analysis of infiltrating immune cells 4 h after application of thioglycolate by FACS. Differentiation of T-cells, granulocytes and monocytes was done by using markers Ly6G and Ly6B.2.

Species

Mouse

Field of application:

Thioglycolate causes a local inflammation after i.p. application. After 4 hours a peritoneal lavage using PBS is done. Lavage fluid is analyzed by FACS and the infiltration of immune cells can be quantified. Lavage fluid can also be analyzed by Multiplex analysis or ELISA. The method can be used to investigate the influence if small molecules or biologicals on cell invasion and on different inflammatory parameters.

The model can be used for the following fields of application:

  • Proof of concept

Endpoints/Outcome parameter

  • Identification of immune cells (Monocytes, Granulocytes, etc.)
  • Quantification of inflammatory parameters (Cytokines, etc.)

Readout parameter

  • Lavage
  • FACS
  • Multiplex
  • ELISA

Quality management and validation

  • Non GLP
  • Controls
  • Randomisation
  • Internal quality management

Weblink(s)

https://www.izi.fraunhofer.de/de/departments/halle-location/department-of-drug-design-and-target-validation/molecular-biotechnology.html

References

  • (1) Cynis et al. The isoenzyme of glutaminyl cyclase is an important regulator of monocyte infiltration under inflammatory conditions. EMBO molecular medicine. 2011; 9: 545–558.