In vitro skin model for the investigation of wound healing promoting substances and wound dressings


Scheme of wound healing in epidermis or full thickness skin model

Species

human

Field of application:

Wound healing is a complex process between cells and their environment initiated upon injury. Many cell types, e.g. thrombocytes, neutrophils, macrophages, lymphocytes, fibroblasts and keratinocytes are involved in the 4 phases of normal wound healing: hemostasis, inflammation, migration-proliferation and remodeling.

A defined wound can be set to our in vitro skin model. To study the biology of normal wound-healing, cell migration, proliferation, enzyme and protein secretion or changes in extracellular matrix, contraction or synthesis of collagen can be investigated. Abnormal healing processes can be simulated using fibroblasts from scars or by adding exudate from chronic wounds.

Endpoints/Outcome parameter

Wound healing-promoting substances and formulations can be topically or systemically applied to the wounded in vitro model. Their potential to support wound healing can be measured with several analytical methods. Wound dressings can be examined with focus on their cell-material or material-extracellular matrix interactions.

Readout parameter

After defined wound setting substances, formulations or wound dressings can be applied. Their potential to promote wound healing can be examined. Different parameters such as viability, proliferation, cytokine profiles, gene expression, interaction of cells with the dressing, can be determined with the following methods

  • Raman-Spectrometry
  • ELISA
  • Flow Cytometry
  • Histology / Immuno-Histology
  • Fluorescence Microscopy
  • Laser Scanning Microscopy
  • Scanning Electron Microscopy

Quality management and validation

SOP, Quality Control, Positive and Negative Control, Reference Substances

References

  • Since 2004, various industrial partners are supported in their development of wound dressings and wound healing-promoting substances.