Xenogeneic tumor model in Nude mice

 

Figure 1: Different noninvasive imaging options for tumors and other applications available at the Fraunhofer IZI.

A: CBCT scan of a mouse. The 2D projections are used to generate a 3D Image.

B+C: MRI of a mouse B shows the abdominal area, in image C the lung and kidneys are visualized.

D: Visualization of a tumor by means of bioluminescence imaging (BLI). Cells are injected into the flank of the mice. Already 7 days later a distinct luminescent signal can be detected.

Species

Mouse

Fields of application

In the xenogeneic murine tumor model cancer cell lines or primary patient cells are injected into immunodeficient Nude mice so that they form a tumor. Human as well as murine cells can be used for this purpose. The tumor can be precisely irradiated after CBCT scan or the scan is used alone for noninvasive imaging. By using cell lines expressing luciferase tumor growth can be noninvasively visualized and quantified by bioluminescence imaging (BLI). Thus the model provides the opportunity to test the potential of novel anti-cancer therapeutics.

  • Pharmacodynamics and pharmacokinetics
  • (Patho)physiological processes
  • Therapeutic efficacy
  • Proof of concept

Endpoints/Outcome parameter

  • Clinical symptoms (tumor growth; in vivo)
  • Hemogram analysis (in vivo)
  • Tumor (patho)histology (ex vivo)

Readout parameter

  • Cone beam computed tomography (CBCT)
  • Measurement of tumor size (by means of caliper)
  • Bioluminescence imaging (BLI)
  • Magnetic resonance imaging (MRI)
  • Animal blood counter
  • Flow cytometric analysis
  • RT-PCR
  • Western Blot
  • Histology (various classical histological stains)
  • Immunohistochemistry

Quality management and validation

  • Controls
  • Blinded induction
  • Blinded data collection and analysis
  • Randomisation
  • Allocation concealment
  • Biometric Expertise
  • Internal quality management

References

  • Commissional work for Tavarlin AG (Darmstadt, Germany). “Inhibition von TKTL1 in einem murinen Tumormodell.”