Species
Mouse
Field of application:
Lipopolysaccharides are an integral component of the outer membrane of gram negative bacteria and cause a systemic inflammation after i.p. application. Various methods (small molecules, etc.) for altering (increase / decrease) inflammatory parameters can be measured.
The model can be used for the following fields of application:
- Inflammation
- Biodistribution
- Proof of concept
Endpoints/Outcome parameter
- Inflammatory parameters (cytokines)
Readout parameter
- BioPlex
- ELISA
- Cytokine Array (membrane based)
- Western Blot
Quality management and validation
- Non GLP
- Controls
- Randomisation
- Blinded data collection and analysis
- Internal quality management
Weblink(s)
https://www.izi.fraunhofer.de/de/departments/halle-location/department-of-drug-design-and-target-validation/molecular-biotechnology.html
References
- Cynis et al. (2011) The isoenzyme of glutaminyl cyclase is an important regulator of monocyte infiltration under inflammatory conditions. EMBO Mol Med 3:545-558