LPS induced inflammation in DBA/1 mice

Pharmacokinetic, in vivo screening
Figure 1: Concentration of CCL2 in blood at 5 different time points after application of different doses of LPS (i.p.), UTC = untreated control.

Species

Mouse

Field of application:

Lipopolysaccharides are an integral component of the outer membrane of gram negative bacteria and cause a systemic inflammation after i.p. application. Various methods (small molecules, etc.) for altering (increase / decrease) inflammatory parameters can be measured.

The model can be used for the following fields of application:

  • Inflammation
  • Biodistribution
  • Proof of concept

Endpoints/Outcome parameter

  • Inflammatory parameters (cytokines)

Readout parameter

  • BioPlex
  • ELISA
  • Cytokine Array (membrane based)
  • Western Blot

Quality management and validation

  • Non GLP
  • Controls
  • Randomisation
  • Blinded data collection and analysis
  • Internal quality management

Weblink(s)

https://www.izi.fraunhofer.de/de/departments/halle-location/department-of-drug-design-and-target-validation/molecular-biotechnology.html

References

  • Cynis et al. (2011) The isoenzyme of glutaminyl cyclase is an important regulator of monocyte infiltration under inflammatory conditions. EMBO Mol Med 3:545-558